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Items for "Western blotting"

Transthyretin amyloidosis in a patient of Iranian-Jewish extraction: a second Israeli-Jewish case

Background: We present a patient with late-onset progressive polyneuropathy and a Congo-red positive staining of sural nerve biopsy, where routine immunohistochemical analysis failed to determine the type of amyloid deposited...

Keywords: familial amyloidotic polyneuropathy, micro-method, mutation V32A, transthyretin amyloid, Western blotting

05/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Detection of antibodies against 60-, 65- and 70-kDa heat shock proteins in paediatric patients with various disorders using Western blotting and ELISA

Background: We examined antibodies against 60-, 65- and 70-kDa heat shock proteins (HSPs) in paediatric healthy individuals, patients with juvenile idiopathic arthritis (JIA) and those undergoing allogeneic stem-cell transplantation for various malignant and non-malignant diseases.

Methods: Western blotting and ELISA were used to examine HSP-directed humoral immune responses.

Results: Using ELISA we detected anti-Hsp60, -Hsp65 and -Hsp70 IgG antibodies in patient sera before, during and after conditioning and at all post-transplant times, as well as in JIA patients and controls...

Keywords: antibody, ELISA, heat shock protein, juvenile idiopathic arthritis, stem cell transplantation, Western blotting

04/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Isolation and biochemical characterization of plasma monoclonal free light chains in amyloidosis and multiple myeloma: a pilot study of intact and truncated forms of light chains and their charge properties

Background: The presence of monoclonal immunoglobulin free light chains (FLC) in the serum is commonly associated with the gammopathies, including multiple myeloma, systemic light chain amyloidosis and non-amyloid light chain deposition disease...

Keywords: amyloidosis, electrophoresis, free light chains, isoelectric focusing, Western blotting

03/2008 | Clinical Chemical Laboratory Medicine, Walter de Gruyter