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Items for "ultracentrifugation"

Interference in a homogeneous assay for low-density lipoprotein cholesterol by lipoprotein X

Background: Homogeneous assays for cholesterol in low-density lipoprotein (LDL) are currently in wide use for guideline-based diagnosis and monitoring of dyslipaemic or coronary conditions...

Keywords: interference, lipoprotein X, low-density lipoprotein cholesterol, negative bias, ultracentrifugation

05/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Use of the TLX ultracentrifuge for the isolation of different density lipoproteins and effects of freeze/thawing of human plasma before ultracentrifugation

Background: Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery.

Methods: We isolated a range of lipoproteins using a preparative ultracentrifuge and the TLX micro-ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand.

Results: In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL...

Keywords: high-density lipoprotein (HDL), lipoproteins, non-HDL cholesterol, small-dense low-density lipoprotein, ultracentrifugation, very low-density lipoprotein

09/2008 | Clinical Chemical Laboratory Medicine, Walter de Gruyter