Items for "trichloroacetic acid"

Strong interference of hemoglobin concentration on CSF total protein measurement using the trichloroacetic acid precipitation method

Background: Among other methods, trichloroacetic acid precipitation is used to quantify total protein in cerebrospinal fluid (CSF).

Methods: We analyzed the influence of hemoglobin on total protein concentration assayed by the trichloroacetic acid method and compared the results to the benzethonium chloride method.

Results: Four CSF samples were spiked with different amounts of hemoglobin, leading to overestimation of protein concentration when assayed by the trichloroacetic acid method...

Keywords: cerebrospinal fluid, hemoglobin, interference, protein, trichloroacetic acid