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Items for "surface plasmon resonance"

Rearrangements in a hydrophobic core region mediate cAMP action in the regulatory subunit of PKA

cAMP-dependent protein kinase (PKA) forms an inactive heterotetramer of two regulatory (R; with two cAMP-binding domains A and B each) and two catalytic (C) subunits...

Keywords: cAMP-dependent protein kinase, exchange protein directly activated by cAMP, surface plasmon resonance

07/2005 | Biological Chemistry, Walter de Gruyter

Determination of the specificity of monoclonal antibodies against Schistosoma mansoni CAA glycoprotein antigen using neoglycoconjugate variants

The immunogenic O-glycan of circulating anodic antigen (CAA) is a high-molecular-mass polysaccharide with the unique ?6)-[?-D-GlcpA-(1?3)]-?-D-GalpNAc-(1? repeating unit...

Keywords: circulating anodic antigen, monoclonal antibody recognition, Schistosoma mansoni, surface plasmon resonance

09/2005 | Biological Chemistry, Walter de Gruyter

?-Calpain binds to lipid bilayers via the exposed hydrophobic surface of its Ca²⁺-activated conformation

?- and m-calpain are cysteine proteases requiring micro- and millimolar Ca²⁺ concentrations for their activation in vitro...

Keywords: acidic loop, C2 domain, Ca2+ requirement, domain V, membrane binding, surface plasmon resonance

05/2006 | Biological Chemistry, Walter de Gruyter

Interaction of the cellular prion protein with raft-like lipid membranes

Conversion of the cellular isoform of the prion protein (PrP^C) into the disease-associated isoform (PrP^Sc) plays a key role in the development of prion diseases...

Keywords: Biacore, GPI anchor, prion, PrPC, surface plasmon resonance

01/2007 | Biological Chemistry, Walter de Gruyter

Comparative thermodynamic analysis of cyclic nucleotide binding to protein kinase A

We have investigated the thermodynamic parameters and binding of a regulatory subunit of cAMP-dependent protein kinase (PKA) to its natural low-molecular-weight ligand, cAMP, and analogues thereof...

Keywords: cAMP-dependent protein kinase, isothermal titration calorimetry, protein-ligand interaction, surface plasmon resonance

02/2007 | Biological Chemistry, Walter de Gruyter

Fast in vitro translation system immobilized on a surface via specific biotinylation of the ribosome

The ribosome is the macromolecular machine responsible for translating the genetic code into polypeptide chains...

Keywords: elongation factor, ribosome, single-molecule fluorescence spectroscopy, surface immobilization, surface plasmon resonance, translation

09/2008 | Biological Chemistry, Walter de Gruyter

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