We determined the zinc binding stoichiometry of the peroxisomal RING-finger proteins by measuring sulphur to metal ratios with ICP-MS coupled to size exclusion chromatography, a strategy that provides a fast and quantitative overview on the binding of metals in proteins...
Keywords: matrix protein import, metal proteins, peroxin, peroxisome biogenesis, PEX2, speciation analysis
01/2007 | Biological Chemistry, Walter de Gruyter