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Items for "sequencing"

The structure of the juvenile hormone binding protein gene from Galleria mellonella

Juvenile hormone (JH) and ecdysone are the key hormones controlling insect growth and development...

Keywords: calycin, carrier protein, evolution, regulatory elements, sequencing

01/2005 | Biological Chemistry, Walter de Gruyter

Determination of the hepatitis C virus subtype: comparison of sequencing and reverse hybridization assays

Background: Hepatitis C virus (HCV) genotyping and accurate subtyping is becoming increasingly relevant to epidemiological studies, clinical management, pathogenicity, and vaccine development.

Methods: The TRUGENE^® HCV 5?NC Genotyping Kit, the new VERSANT^® HCV Genotype 2.0 Assay (LiPA), and a new laboratory-developed HCV NS5b sequencing assay designed for automated sequencing of the HCV NS5b region were used...

Keywords: hepatitis C virus, NS5b, reverse hybridization, sequencing, subtype

02/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

External and internal fungal flora of pine sapwood (Pinus sylvestris L.) specimens in above-ground field tests at six different sites in south-west Germany

The occurrence of fungal species on pine sapwood samples obtained from an above-ground field test study was analysed by terminal restriction fragment length polymorphism (T-RFLP), cloning, and sequencing...

Keywords: decay, double layer, Fungi, sequencing, service life prediction, soft rot, T-RFLP

01/2007 | Holzforschung, Walter de Gruyter

Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi

In the present work PCR technology was used as a tool to detect the early stages of wood decay and was compared with microscopic evaluation...

Keywords: brown rot, Coniophora, fungal colonisation, Fungi, polymerase chain reaction (PCR), sequencing, terminal restriction fragment length polymorphism (T-RFLP), wood