Items for "reverse transcriptase-polymerase chain reaction (RT-PCR)"

Combined androgen blockade therapy can convert RT-PCR detection of prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSMA) transcripts from positive to negative in the peripheral blood of patients with clinically localized prostate c

Background: The clinical relevance of positive molecular staging as defined by reverse transcriptase-polymerase chain reaction (RT-PCR) detections of both prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSMA) transcripts in the peripheral blood (PB) of patients with prostate cancer is still debatable.

Methods: We analyzed the biochemical failure-free survival (bFFS) of prostate cancer patients with positive molecular staging who underwent immediate curative therapy (Group I, n=39) compared to prostate cancer patients who did convert their positive molecular staging by the administration of combined androgen blockade (CAB) for 12 months prior to curative treatment (Group II, n=15).

Results: The median bFFS for Group I was 9 months (95% CI 5–13 months) and was significantly lower compared to Group II (>36 months, p<0.001)...

Keywords: combined androgen blockade, pre-operative risk stratification, prostate cancer, prostate-specific antigen (PSA), prostate-specific membrane antigen (PSMA), reverse transcriptase-polymerase chain reaction (RT-PCR)