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recombinant expression
Items for "recombinant expression"
cDNA cloning and heterologous expression of a wheat proteinase inhibitor of subtilisin and chymotrypsin (WSCI) that interferes with digestive enzymes of insect pests
A cDNA encoding the proteinase inhibitor WSCI (wheat subtilisin/chymotrypsin inhibitor) was isolated by RT-PCR...
Keywords: cDNA cloning, E. coli, insect proteinases, proteinase inhibitor, recombinant expression, wheat proteinase
04/2005 | Biological Chemistry, Walter de GruyterEfficient production of native actin upon translation in a bacterial lysate supplemented with the eukaryotic chaperonin TRiC
Recombinant expression of actin in bacteria results in non-native species that aggregate into inclusion bodies...
Keywords: molecular chaperones, protein folding, recombinant expression
08/2005 | Biological Chemistry, Walter de GruyterPurification and characterization of natural Ara h 8, the Bet v 1 homologous allergen from peanut, provides a novel isoform
The peanut allergen Ara h 8 is an important allergen for birch pollen allergic patients because of the cross-reactivity to the homologous Bet v 1...
Keywords: Ara h 8, peanut allergy, PR-10 protein, purification, recombinant expression, trypsin fingerprinting
04/2008 | Biological Chemistry, Walter de Gruyter