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Items for "real-time PCR"

Expression of the human kallikrein genes 10 (KLK10) and 11 (KLK11) in cancerous and non-cancerous lung tissues

Only one transcript for KLK10 was identified by RT-PCR in lung tissue, whereas KLK11 expressed at least four alternative transcripts...

Keywords: bronchus, Lung Cancer, real-time PCR, serine protease, tissue kallikrein-related protein

06/2006 | Biological Chemistry, Walter de Gruyter

Comparison of the Roche COBAS Amplicor? Monitor, Roche COBAS Ampliprep?/COBAS Taqman? and Abbott RealTime? Test assays for quantification of hepatitis C virus and HIV RNA

Background: We have evaluated the performance of two newly developed automated real-time PCR assays, the COBAS Ampliprep?/COBAS TaqMan? (CAP/CTM) and the Abbott RealTime tests, in the quantification of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) RNA...

Keywords: automation, hepatitis C virus (HCV), human immunodeficiency virus (HIV), quantification, real-time PCR

07/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

An Italian program of external quality control for quantitative assays based on real-time PCR with Taq-Man? probes

Quantitative real-time PCR techniques are increasingly being used for the measurement of nucleic acids in research applications as well as in the clinical laboratory...

Keywords: fluorogenic probes, human telomerase reverse transcriptase (hTERT), real-time PCR, real-time platforms

05/2005 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Rapid detection of the factor XIII Val34Leu (163 G?T) polymorphism by real-time PCR using fluorescence resonance energy transfer detection and melting curve analysis

The Val34Leu polymorphism in the A subunit of blood coagulation factor XIII (FXIII-A) is located in the activation peptide, just three amino acids upstream of the thrombin cleavage site...

Keywords: factor XIII, fluorescence resonance energy transfer, gene polymorphism, real-time PCR

08/2004 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Bivariate statistical approach to evaluate laboratory performance by analysis of standard curves in an External Quality Assurance program for quantitative assays based on real-time PCR with Taq-Man? probes

Recently a revolutionary technique for quantitative PCR determination was introduced in diagnostic laboratories...

Keywords: bivariate analysis, EQA program, real-time PCR, standard curves

01/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Increase in and clearance of cell-free plasma DNA in hemodialysis quantified by real-time PCR

Background: Recently cell-free plasma DNA has been described as a marker of apoptosis during hemodialysis (HD), but little is known about how different dialysis membranes may contribute to this process or whether pre-HD levels are restored afterwards...

Keywords: Apoptosis, cell-free plasma DNA, circulating DNA, Dialysis membrane, Hemodialysis, real-time PCR, renal insufficiency

12/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Comparison of four commercial quantitative HIV-1 assays for viral load monitoring in clinical daily routine

Background: Quantification of viral load (VL) is standard for monitoring HIV-1 therapy and is crucial before deciding whether to switch or to continue a current antiretroviral regimen.

Methods: We compared the performance of the four most widely used commercial viral-load assays, COBAS Amplicor Monitor v1.5, Versant HIV-1 RNA 3.0, Abbott RealTime HIV-1 and Cobas AmpliPrep/Cobas TaqMan HIV-1 (CAP/CTM), in terms of intra- and inter-assay variability, as well as hands-on-time, specificity and ability to quantify group M subtypes.

Results: Although linearity and correlation were confirmed for the assays and comparable sensitivity and specificity were verified for genetically diverse HIV-1 subtypes, demonstrating suitability for monitoring of HIV group M isolates, the viral loads obtained showed variations, with a mean difference of 0.1–0.4 log, depending on the system used.

Conclusions: Although sensitivity and precision were confirmed for all the systems, differences between them should be taken into account when viral load monitoring of the same person is performed using different systems.

Clin Chem Lab Med 2007;45:93–9.

Keywords: HIV-1, monitoring, quantification, real-time PCR, viral load

01/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Genotyping of the LCT _(T/C-13910) polymorphism on the LightCycler using fluorescent hybridisation probes Genotypisierung des LCT _(T/C-13910)-Polymorphismus am LightCycler mittels fluoreszenzmarkierter Hybridisierungssonden

Primary adult-type hypolactasia is a hereditary metabolic condition which leads to clinical manifestation usually during early adulthood and is frequently associated with clinical symptoms of lactose intolerance...

Keywords: Laktose-H2-Atemtest, LCT-Genotyp, Primärer adulter Laktasemangel, Real-time PCR

07/2005 | LaboratoriumsMedizin, Walter de Gruyter

Real-time NAT-based methods for detection of bacterial contamination in blood products

Bacterial contamination of blood components, especially of platelet concentrates (PCs), represents the highest infectious risk in blood transfusion...

Keywords: bakterielle Kontamination, Real-time PCR, Standardisierung, Sterilitätstestung

12/2006 | LaboratoriumsMedizin, Walter de Gruyter

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You are here: Home :: Keyword index :: ray bis sof :: ray microbuckling - real-time RT-PCR real-time PCR

Items for "real-time PCR"