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Items for "quantification"

Fluorimetric quantitation of citalopram and escitalopram in plasma: developing an express method to monitor compliance in clinical trials

Background: Selective serotonin reuptake inhibitors (SSRIs) in general, and citalopram/escitalopram in particular, are widely used to treat clinical depression...

Keywords: citalopram, drug plasma levels, escitalopram, fluorimetry, quantification

04/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Comparison of the Roche COBAS Amplicor? Monitor, Roche COBAS Ampliprep?/COBAS Taqman? and Abbott RealTime? Test assays for quantification of hepatitis C virus and HIV RNA

Background: We have evaluated the performance of two newly developed automated real-time PCR assays, the COBAS Ampliprep?/COBAS TaqMan? (CAP/CTM) and the Abbott RealTime tests, in the quantification of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) RNA...

Keywords: automation, hepatitis C virus (HCV), human immunodeficiency virus (HIV), quantification, real-time PCR

07/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Comparison of four commercial quantitative HIV-1 assays for viral load monitoring in clinical daily routine

Background: Quantification of viral load (VL) is standard for monitoring HIV-1 therapy and is crucial before deciding whether to switch or to continue a current antiretroviral regimen.

Methods: We compared the performance of the four most widely used commercial viral-load assays, COBAS Amplicor Monitor v1.5, Versant HIV-1 RNA 3.0, Abbott RealTime HIV-1 and Cobas AmpliPrep/Cobas TaqMan HIV-1 (CAP/CTM), in terms of intra- and inter-assay variability, as well as hands-on-time, specificity and ability to quantify group M subtypes.

Results: Although linearity and correlation were confirmed for the assays and comparable sensitivity and specificity were verified for genetically diverse HIV-1 subtypes, demonstrating suitability for monitoring of HIV group M isolates, the viral loads obtained showed variations, with a mean difference of 0.1–0.4 log, depending on the system used.

Conclusions: Although sensitivity and precision were confirmed for all the systems, differences between them should be taken into account when viral load monitoring of the same person is performed using different systems.

Clin Chem Lab Med 2007;45:93–9.

Keywords: HIV-1, monitoring, quantification, real-time PCR, viral load

01/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Screening fungi tolerant to Western red cedar (Thuja plicata Donn) extractives. Part 1. Mild extraction by ultrasonication and quantification of extractives by reverse-phase HPLC

Western red cedar (Thuja plicata Donn) heartwood samples were extracted in methanol...

Keywords: extractives, HPLC, lignan, quantification, tropolone, Western red cedar (Thuja plicata Donn)

03/2007 | Holzforschung, Walter de Gruyter

Determination of ethylenic residues in wood and TMP of spruce by FT-Raman spectroscopy

A method based on FT-Raman spectroscopy is proposed for determining in situ concentrations of ethylenic residues in softwood lignin...

Keywords: coniferaldehyde, coniferyl alcohol, DHP, ethylenic residues, lignin, quantification, Raman, thermomechanical pulp, wood

11/2008 | Holzforschung, Walter de Gruyter

Critical evaluation of ¹H NMR metabonomics of serum as a methodology for disease risk assessment and diagnostics

This review focuses on (i) the current status of ¹H NMR spectroscopy to quantify lipoprotein subclasses directly from serum or plasma, and (ii) the applications of ¹H NMR metabonomics of serum in biomedicine...

Keywords: blood plasma and serum, coronary heart disease, data analysis, diagnostics, lipoprotein subclass, metabolomics, metabonomics, nuclear magnetic resonance spectroscopy, quantification, Risk assessment

01/2008 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

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