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Items for "Pyrosequencing?"

The vegetarian lifestyle and DNA methylation

Vegetarians have a lower intake of vitamin B12 than omnivores do. Vitamin B12 deficiency (holotranscobalamin II <35 pmol/L or methylmalonic acid >271 nmol/L) was found in 58% of 71 vegetarians studied...

Keywords: DNA methylation, homocysteine, Pyrosequencing?, vegetarians, vitamin B12

10/2005 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Simultaneous genotyping of the three lactose tolerance-linked polymorphisms LCT –13907C>G, LCT –13910C>T and LCT –13915T>G with Pyrosequencing? technology

Background: We required a new genotyping method for the diagnosis of adult hypolactasia, which would allow the simultaneous genotyping of three known polymorphic loci linked to lactose tolerance (LCT –13907C>G, LCT –13910C>T and LCT –13915T>G) in a single PCR/pyrosequencing test run.

Method: We utilized Pyrosequencing? technology, a DNA-sequence-by-synthesis technique.

Results: The developed Pyrosequencing? method allowed genotyping of the three loci LCT –13907C>G, LCT –13910C>T and LCT –13915T>G in a single PCR/pyrosequencing test run...

Keywords: adult hypolactasia, Africans, Arabs, lactose tolerance, Pyrosequencing?

01/2008 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Decreased p66Shc promoter methylation in patients with end-stage renal disease

Background: p66Shc is a stress response protein and partially regulated by epigenetic modifications...

Keywords: atherosclerosis, DNA methylation, homocysteine, p66Shc, Pyrosequencing?

12/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter