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Items for "purification"

Purification of Mg²⁺-dependent phosphatidate phosphohydrolase from rat liver: new steps and aspects

A new procedure for the partial purification of Mg²⁺-dependent, N-ethylmaleimide-sensitive phosphatidate phosphohydrolase (Mg²⁺-PAP; EC 3.1.3.4) from rat liver cytosol is described, using protein precipitation with MgCl₂, gel filtration on Sephacryl S-400, chromatography on DEAE-cellulose and affinity chromatography on calmodulin-agarose...

Keywords: 90-kDa heat shock protein ?-isoform, purification, rat liver cytosol

11/2005 | Biological Chemistry, Walter de Gruyter

Glutamic acid-141: a heme ‘bodyguard’ in anionic tobacco peroxidase

The role of the conserved glutamic acid residue in anionic plant peroxidases with regard to substrate specificity and stability was examined...

Keywords: expression, purification, radiation-induced inactivation, recombinant tobacco peroxidase, refolding, site-directed mutagenesis, substrate specificity

04/2007 | Biological Chemistry, Walter de Gruyter

The antibacterial compound sulphoglycerolipid 1-0 palmitoyl-3-0(6?-sulpho-?-quinovopyranosyl)-glycerol from Sargassum wightii Greville (Phaeophyceae)

The compound sulphoglycerolipid 1-0-palmitoyl-3-0(6?-sulpho-?-quinovopyranosyl)-glycerol isolated from the methanolic extract of the brown seaweed Sargassum wightii is active against Xanthomonas oryzae pv...

Keywords: antibacterial compound, purification, Sargassum wightii, seaweed, sulphoglycerolipid, Xanthomonas oryzae pv. oryzae

12/2005 | Botanica Marina, Walter de Gruyter

Purification and characterization of natural Ara h 8, the Bet v 1 homologous allergen from peanut, provides a novel isoform

The peanut allergen Ara h 8 is an important allergen for birch pollen allergic patients because of the cross-reactivity to the homologous Bet v 1...

Keywords: Ara h 8, peanut allergy, PR-10 protein, purification, recombinant expression, trypsin fingerprinting

04/2008 | Biological Chemistry, Walter de Gruyter

Fractionation and characterization of xylan rich extracts from birch

There is a need for efficient separation processes that provide clean fractions of individual wood polymers...

Keywords: bonded lignin, fractionation, lignin-carbohydrate complexes, Liquid-liquid extraction, purification, Solubility, unbounded lignin, xylan

01/2008 | Holzforschung, Walter de Gruyter

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