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Items for "proteinase inhibitor"

cDNA cloning and heterologous expression of a wheat proteinase inhibitor of subtilisin and chymotrypsin (WSCI) that interferes with digestive enzymes of insect pests

A cDNA encoding the proteinase inhibitor WSCI (wheat subtilisin/chymotrypsin inhibitor) was isolated by RT-PCR...

Keywords: cDNA cloning, E. coli, insect proteinases, proteinase inhibitor, recombinant expression, wheat proteinase

04/2005 | Biological Chemistry, Walter de Gruyter
Modeling the 3D structure of wheat subtilisin/chymotrypsin inhibitor (WSCI). Probing the reactive site with two susceptible proteinases by time-course analysis and molecular dynamics simulations

Comparative modeling and time-course hydrolysis experiments have been applied to investigate two enzyme-inhibitor complexes formed between the wheat subtilisin-chymotrypsin inhibitor (WSCI) and two susceptible proteinases...

Keywords: MALDI-TOF mass spectrometry, molecular simulations, proteinase inhibitor, proteinase-inhibitor complex, reactive site, recombinant WSCI

07/2006 | Biological Chemistry, Walter de Gruyter