Items for "protein design"

Rational engineering of a fluorescein-binding anticalin for improved ligand affinity

The anticalin FluA is an artificial lipocalin with novelspecificity for the fluorescein group, which was engineered from an insect bilin-binding protein by targeted random mutagenesis and selection...

Keywords: circular dichroism, fluorescence titration, hapten, ligand binding, lipocalin, protein design, Spectroscopy