You are here: Home :: Keyword index :: par bis pre :: Polyanionic compound - Polymethylmethacrylate polymerase chain reaction

Items for "polymerase chain reaction"

Reference materials (RMs) for analysis of the human factor II (prothrombin) gene G20210A mutation

The Scientific Committee of Molecular Biology Techniques (C-MBT) in Clinical Chemistry of the IFCC has initiated a joint project in co-operation with the European Commission, Joint Research Centre, Institute of Reference Materials and Measurements to develop and produce plasmid-type reference materials (RMs) for the analysis of the human prothrombin gene G20210A mutation...

Keywords: metrological traceability, molecular diagnostic testing, mutation, polymerase chain reaction, prothrombin, recombinant DNA, reference standards

08/2005 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Molecular detection of tyrosinase transcripts in peripheral blood from patients with malignant melanoma: correlation of PCR sensitivity threshold with clinical and pathologic disease characteristics

Background: Positive molecular detection of tyrosinase transcripts (TYR mRNA) in RNA extracts of peripheral blood (PB) samples from patients with malignant melanoma provides evidence of disease dissemination.

Methods: Total RNA extracted from PB was quantified and subjected to RT-PCR under ultra-sensitive and reduced-sensitivity PCR conditions using SSRT-II...

Keywords: Malignant melanoma, mRNA, peripheral, polymerase chain reaction, tyrosinase

12/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Multiplex polymerase chain reaction on FTA cards vs. flow cytometry for B-lymphocyte clonality

Background: Two-colour flow cytometry was compared with multiplex PCR with capillary electrophoresis for clonality determination in specific categories of B-cell lymphoma...

Keywords: B-cell, comparative study, DNA/analysis/isolation and purification, flow cytometry, lymphoma, polymerase chain reaction

03/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Cervical screening in the 21st century: the case for human papillomavirus testing of self-collected specimens

Cervical screening by Pap smear involves a high rate of false negatives, necessitating frequent testing...

Keywords: cervical cancer, cervical screening, human papillomavirus, hybrid capture, polymerase chain reaction, specimen self-collection, tampon

05/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Use of maternal plasma for non-invasive prenatal diagnosis of fetal ABO genotypes

Background: Measurement of free fetal DNA in maternal plasma opened a door for non-invasive prenatal diagnosis...

Keywords: ABO blood group system, maternal plasma, polymerase chain reaction, prenatal diagnosis

08/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter