Items for "POD-like activity"

Role of methionine residues of albumin in T-R conversion of hemoglobin

Background: Hemoglobin (Hb) has peroxidase (POD)-like activity. We found that the addition of albumin to human Hb purified from venous erythrocytes increased its POD-like activity.

Methods: We treated human Hb with a Blue-Toyopearl column of immobilized albumin, compared the treated Hb and native Hb for POD-like activity, the level of Hb-bound 2,3-diphosphoglycerate (2,3-DPG), and the absorption spectrum, and found that treatment with albumin released 2,3-DPG from Hb, resulting in a tense-relaxed (T-R) conversion and increased POD-like activity.

Results: The addition of human, mouse, rat, or bovine albumin to human Hb increased its POD-like activity...

Keywords: Albumin, domain, domain I, hemoglobin, Methionine, POD-like activity