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Items for "PCR"

mRNA expression analysis of human kallikrein 11 (KLK11) may be useful in the discrimination of benign prostatic hyperplasia from prostate cancer after needle prostate biopsy

Kallikrein 11 (KLK11, TLSP, hippostatin) is a member of the human kallikrein gene family, which includes PSA, KLK2 and 12 other members, all localized on chromosome 19q13.4...

Keywords: free PSA, kallikreins, PCR, prostate, prostate cancer, prostate-specific antigen, PSA, serine proteases, tumor biomarkers

06/2006 | Biological Chemistry, Walter de Gruyter
Mid-region parathyroid hormone-related protein (PTHrP) and gene expression of MDA-MB231 breast cancer cells

We have previously shown that PTHrP(38–94) amide restrains growth and invasion in vitro, causes striking toxicity and accelerates death of some breast cancer cell lines, the most responsive being MDA-MB231, for which tumorigenesis was also attenuated in vivo...

Keywords: breast cancer, differential display, Gene expression, PCR, PTHrP

05/2007 | Biological Chemistry, Walter de Gruyter
Cervical human papillomavirus screening by PCR: advantages of targeting the E6/E7 region

PCR is a promising method for detection of human papillomavirus (HPV), the high-risk forms of which are responsible for cervical cancer...

Keywords: cervical cancer, cervical dysplasia, cervical intrepithelial neoplasia (CIN), diagnostic testing, oncogenes, open reading frames E6, E7, E1, E2 and L1, papillomavirus, human, PCR, viral integration

11/2005 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Detection rate of Helicobacter pylori stool antigen in newborn infants and small children

Aims: To investigate the prevalence of H. pylori antigen in the stools of Norwegian neonates and small children.

Methods: A total of 249 children aged 0 days–3 years were tested for the presence of H...

Keywords: Helicobacter, HpSA, infants, infection, Norway, PCR, perinatal, prevalence, stool, transient colonization

04/2007 | Journal of Perinatal Medicine, Walter de Gruyter
Zur Labordiagnostik von Entamoeba histolytica-Infektionen. The laboratory diagnosis of Entamoeba histolytica-infections

Zusammenfassung

Zusammenfassung Die klassische Stuhlmikroskopie zum Nachweis intestinaler Protozoen-Infektionen wird mehr und mehr ersetzt durch neue Detektionsverfahren wie Immunfluoreszenz, Antigen-ELISA oder PCR...

Keywords: Antigen-ELISA, Diagnostik, Entamoeba histolytica, mikroskopische Stuhluntersuchung, PCR, Serologie, diagnosis, microscopic stool examination, serology

12/2004 | LaboratoriumsMedizin, Walter de Gruyter
Zur Diagnostik der Leishmania-Infektionen. Diagnosis of Leishmania infections

Zusammenfassung

Zusammenfassung Mehr als 20 Leishmanien-Spezies sind für den Menschen pathogen...

Keywords: Anzucht, Kala Azar, Leishmaniosen, Mikroskopie, Orientbeule, PCR, culture, leishmaniases, microscopy, oriental sore

12/2004 | LaboratoriumsMedizin, Walter de Gruyter
Richtlinie Molekulare Erregerdiagnostik Update 2007 / Guideline Molecular Diagnostics of Pathogens Update 2007

Zusammenfassung Die Arbeitsgruppe Molekulare Erregerdiagnostik der sterreichischen Gesellschaft fr Gute Analysen- und Laborpraxis hat erstmals im Jahr 1998 eine Richtlinie zur molekularen Erregerdiagnostik erstellt, die im Jahr 2001 aktualisiert wurde...

Keywords: IVD, molekulare Erregerdiagnostik, sterreich, PCR, Richtlinie, Routinelabor, Austria, Guideline, molecular diagnostics, pathogens, routine laboratory

04/2007 | LaboratoriumsMedizin, Walter de Gruyter
DNA microarrays in microbiology1

The techniques for cultivation and identification of micro-organisms have changed little since the emergence of microbiology as a distinct discipline just over a hundred years ago...

Keywords: antibiotic resistance, bacteriology, DNA microarray, PCR

09/2008 | LaboratoriumsMedizin, Walter de Gruyter
Einsatz der Genchiptechnologie in der Erregerdiagnostik / Use of DNA microarrays in microbiological diagnostics

Zusammenfassung Seit der Geburt der Mikrobiologie vor etwas mehr als 100 Jahren hat sich die technische Grundlage der Keimzüchtung und -identifizierung kaum geändert...

Keywords: Bakteriologie, DNA-Microarray, Genchip, PCR, Resistenzbestimmung, antibiotic resistance, bacteriology, DNA microarray

09/2008 | LaboratoriumsMedizin, Walter de Gruyter
Diagnostik und Resistenztestung bei Pneumocystis jirovecii / Diagnostic features and resistance testing of Pneumocystis jirovecii

Zusammenfassung Die durch den Pilz Pneumocystis jirovecii (P. jirovecii) ausgelöste Pneumocystis Pneumonie (PCP) ist vor allem bei HIV-Infizierten bekannt...

Keywords: Co-trimoxazol, PCP, PCR, Pneumocystis, Resistenz, Sulfamethoxazol, Trimethoprim, co-trimoxazole, Pneumocystis Pneumonia (PCP), Resistance, sulfamethoxazole

01/2008 | LaboratoriumsMedizin, Walter de Gruyter
Diagnostic features and resistance testing for Pneumocystis jirovecii1

Pneumocystis pneumonia (PCP), caused by the fungus Pneumocystis jirovecii (P...

Keywords: co-trimoxazole, PCP, PCR, Pneumocystis, Resistance, sulfamethoxazole, Trimethoprim

01/2008 | LaboratoriumsMedizin, Walter de Gruyter
Aktuelle Verfahren zum Nukleinsäure gestützten Direktnachweis von MRSA / Direct nucleic acid-based detection of MRSA in clinical specimens

Zusammenfassung In den letzten Jahren ist weltweit eine dramatische Zunahme der Methicillin-Resistenz bei Staphylococcus aureus-Isolaten zu beobachten...

Keywords: Direktnachweis, mecA, Methicillin-resistente Staphylococcus aureus, MRSA, PCR, SCCmec, Direct detection, Methicillin-resistant Staphylococcus aureus

07/2008 | LaboratoriumsMedizin, Walter de Gruyter
Direct nucleic acid-based detection of MRSA from clinical specimens1

Over the past few years a dramatic increase in the methicillin resistance of S. aureus isolates has been observed worldwide...

Keywords: Direct detection, mecA, Methicillin-resistant Staphylococcus aureus, MRSA, PCR, SCCmec

07/2008 | LaboratoriumsMedizin, Walter de Gruyter
Rapid methods for diagnosis of bloodstream infections

Direct detection technologies for pathogenic microorganisms are emerging to be applied in the diagnosis of serious bloodstream infections and infections at sterile body sites, as well as for quality control measures prior to the release of sterile blood products and to ascertain microbial safety of food...

Keywords: bacteraemia, blood culture, flow cytometry, hybridisation, matrix-assisted laser-desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF), microarray, microbial food safety, nucleic acid-based technology (NAT), nucleic acid sequence based amplification (NASBA), PCR, proteomic profiling, sepsis

07/2008 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Mother-child double incompatibility at vWA and D5S818 loci in paternity testing

Background: In a paternity dispute case, 17 autosomal short tandem repeats (STR) were examined and signified a possible paternal mismatch at vWA locus and a maternal mismatch at D5S818 locus in the child under investigation.

Methods: Seventeen autosomal, 17 Y-chromosome and six X-chromosome repeat loci were used in parentage analysis...

Keywords: DNA sequencing, DNA typing, mismatch, paternity/maternity index, paternity test, PCR, short tandem repeats (STR), X-STR, Y-STR

10/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter