Items for "online solid-phase extraction-liquid chromatography-tandem mass spectrometry (XLC-MS/MS)"

Direct and fast determination of antiretroviral drugs by automated online solid-phase extraction-liquid chromatography-tandem mass spectrometry in human plasma

Background: In this study antiretroviral drugs of the classes protease inhibitors (PI) and non-nucleoside reverse transcriptase inhibitors (NNRTI) were quantified for the first time directly in patient plasma samples by means of an automated and validated online solid-phase extraction-liquid chromatography-tandem mass spectrometry (XLC-MS/MS) method using the Symbiosis Pharma^® system (Spark Holland) for XLC coupled to an API 2000 for MS/MS analysis.

Methods: The PI drugs amprenavir, nelfinavir, indinavir, lopinavir, saquinavir, ritonavir, and atazanavir, and the NNRTI drugs nevirapine and efavirenz in real patient samples were analysed in a 25-?L sample volume, which was only diluted with 200 ?L of H₂O (containing 500 ng/mL of the internal standard reserpine) to minimise the matrix concentration and to add the internal standard...

Keywords: antiretroviral drug, direct plasma analysis, online SPE, online solid-phase extraction-liquid chromatography-tandem mass spectrometry (XLC-MS/MS), Symbiosis Pharma® system