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A method to determine RNA and DNA oxidation simultaneously by HPLC-ECD: greater RNA than DNA oxidation in rat liver after doxorubicin administration

We developed a novel method for the simultaneous extraction and analysis of total tissue RNA and DNA to quantify the RNA and DNA oxidation products 8-oxo-7,8-dihydroguanosine and 8-oxo-7,8-dihydro-2?-deoxyguanosine using HPLC coupled to electrochemical detection (HPLC-ECD)...

Keywords: doxorubicin, free radical, nucleic acids, oxidative stress, 8-oxodGuo, 8-oxoGuo

01/2006 | Biological Chemistry, Walter de Gruyter

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You are here: Home :: Keyword index :: nad bis par :: Nuclear counting - Nucleophilic aromatic substitution nucleic acids

Items for "nucleic acids"

A method to determine RNA and DNA oxidation simultaneously by HPLC-ECD: greater RNA than DNA oxidation in rat liver after doxorubicin administration