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Items for "N-acetyl-?-D-hexosaminidase"

Optimization of an enzymatic method for the determination of lysosomal N-acetyl-?-D-hexosaminidase and ?-glucuronidase in synovial fluid

Background: Our goal was to develop a suitably sensitive assay for N-acetyl-?-D-hexosaminidase (HEX) and ?-glucuronidase to allow their use as markers of joint diseases.

Methods: We optimized a spectrophotometric method for the determination of lysosomally derived HEX and ?-glucuronidase in synovial fluid on a microplate reader to improve its utility...

Keywords: ?-glucuronidase, Km, N-acetyl-?-D-hexosaminidase, rheumatoid arthritis, synovial fluid

08/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Fluorimetric determination of activity and isoenzyme composition of N-acetyl-?-D-hexosaminidase in seminal plasma of fertile men and infertile patients with secretory azoospermia

Background: The activity and isoenzyme composition of N-acetyl-?-D-hexosaminidase (EC.3.2.1.52) in seminal plasma of fertile and infertile men have been evaluated...

Keywords: N-acetyl-?-D-hexosaminidase, fluorimetric determination, isoenzyme, male infertility, receiver operating characteristic curve analysis, secretory azoospermia, seminal plasma

07/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter