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Items for "mutagenesis"

Detailed analysis of MIA protein by mutagenesis

MIA (melanoma inhibitory activity) has been identified as a small protein secreted by malignant melanoma cells that interacts with extracellular matrix proteins including fibronectin...

Keywords: melanoma, melanoma inhibitory activity, MIA function, mutagenesis

12/2006 | Biological Chemistry, Walter de Gruyter

Cancer-preventive anti-oxidants that attenuate free radical generation by inflammatory cells

Active inflammatory leukocytes are a major endogenous source of reactive oxygen and nitrogen oxide species (RONS)...

Keywords: chemoprevention, co-culture, macrophage, mutagenesis, nitric oxide, phytochemicals, superoxide

04/2006 | Biological Chemistry, Walter de Gruyter

Conformational studies on Arabidopsis sulfurtransferase AtStr1 with spectroscopic methods

Sulfurtransferases/rhodaneses (Str) are enzymes widely distributed in archaea, prokaryota and eukaryota, and catalyze the transfer of sulfur from a donor molecule to a thiophilic acceptor substrate...

Keywords: Arabidopsis thaliana, circular dichroism, 3-mercaptopyruvate, mutagenesis, thiosulfate, tryptic digestion

01/2007 | Biological Chemistry, Walter de Gruyter

Characterization of the large subunit of EcoHK31I methyltransferase by structural modeling and mutagenesis

M.EcoHK31I is a naturally occurring mC5-methyltransferase with a large ? polypeptide and a small ? polypeptide...

Keywords: EcoHK31I, Escherichia coli, methyltransferase, mutagenesis, restriction-modification system

03/2007 | Biological Chemistry, Walter de Gruyter

Characterization of peptidyl-tRNA hydrolase encoded by open reading frame Rv1014c of Mycobacterium tuberculosis H37Rv

The enzyme peptidyl-tRNA hydrolase (Pth, EC 3.1.1.29) is essential for the viability of bacteria...

Keywords: complementation, enzyme activity, molecular modeling, mutagenesis, peptidyl-tRNA hydrolase, thermal unfolding

05/2007 | Biological Chemistry, Walter de Gruyter

Annexin B1 from Taenia solium metacestodes is a newly characterized member of the annexin family

We previously reported cloning of the Taenia solium annexin B1 gene from a metacestode cDNA expression library and demonstrated that it acts as a protective antigen for effective vaccine development against cysticercosis...

Keywords: calcium binding, CD spectroscopy, mutagenesis, phospholipid binding, Taenia solium

06/2007 | Biological Chemistry, Walter de Gruyter

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