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Items for "mRNA"

Comparative proteomic analysis of neoplastic and non-neoplastic germ cell tissue

A comparative proteomic analysis of neoplastic versus non-neoplastic seminoma identified glutathione S-transferase M3 as a differentially expressed protein...

Keywords: 2D-PAGE, glutathione S-transferase M3, Mass spectrometry, mRNA, proteome, testicular neoplasm

04/2006 | Biological Chemistry, Walter de Gruyter

Molecular detection of tyrosinase transcripts in peripheral blood from patients with malignant melanoma: correlation of PCR sensitivity threshold with clinical and pathologic disease characteristics

Background: Positive molecular detection of tyrosinase transcripts (TYR mRNA) in RNA extracts of peripheral blood (PB) samples from patients with malignant melanoma provides evidence of disease dissemination.

Methods: Total RNA extracted from PB was quantified and subjected to RT-PCR under ultra-sensitive and reduced-sensitivity PCR conditions using SSRT-II...

Keywords: Malignant melanoma, mRNA, peripheral, polymerase chain reaction, tyrosinase

12/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Gene expression studies with microarrays and SAGE: biological and analytical principles1

The present article continues a series of publications and internet presentations (www.dgkl.de/bioninformatik) on analytical and postanalytical aspects of gene expression studies...

Keywords: DNA microarrays, Gene expression profiling, mRNA, SAGE

09/2008 | LaboratoriumsMedizin, Walter de Gruyter

Genexpressionsstudien mit Microarrays und SAGE: Biologische und analytische Grundlagen / Gene expression studies with microarrays and SAGE: biological and analytical principles

Zusammenfassung Diese Arbeit setzt eine im Jahr 2003 begonnene Publikationsserie über die Analytik und Auswertung von Genexpressionsstudien der Arbeitsgruppe Bioinformatik der DGKL e.V...

Keywords: DNA-Microarrays, Genexpressionsprofile, mRNA, SAGE, DNA microarrays, Gene expression profiling, serial analysis of gene expression (SAGE)