We determined the zinc binding stoichiometry of peroxisomal RING finger proteins by measuring sulfur/metal ratios using inductively coupled plasma-mass spectrometry coupled to size exclusion chromatography, a strategy that provides a fast and quantitative overview on the binding of metals in proteins...
Keywords: matrix protein import, metal proteins, peroxin, peroxisome biogenesis, PEX2, speciation analysis
11/2007 | Biological Chemistry, Walter de Gruyter