Items for "Mass spectrometry"

Identification of a new truncated form and deamidation products of fibrinopeptide B released by thrombin from human fibrinogen

Quantification of fibrinopeptides release is widely used to investigate fibrinogen activation, and standard chromatographic or capillary electrophoretic procedures are readily available...

Keywords: blood coagulation, human, fibrinogen, fibrinopeptides, chromatography, Mass spectrometry

Preparation and characterization of working standards for ²³⁸Pu

An attempt is made to prepare and characterize the working standards
of ²³⁸Pu by mixing solutions of enriched isotopes of
²³⁸Pu and ²³⁹Pu, available indigenously...

Keywords: Alpha spectrometry, 238Pu, Mass spectrometry, Working standards, Quality assurance

Lysophosphatidic acid affinity chromatography reveals pyruvate kinase as a specific LPA-binding protein

Lysophosphatidic acid is a pleiotropic lipid signalingmolecule that evokes a broad array of cellular responses including proliferation, tumor cell invasion, neurite retraction, cytoskeletal rearrangements and smooth muscle contraction...

Keywords: circular dichroism, enzymatic activity, gel filtration, isothermal calorimetry titration, Mass spectrometry, phospholipid binding

First identification of a phosphorylcholine-substituted protein from Caenorhabditis elegans: isolation and characterization of the aspartyl protease ASP-6

Caenorhabditis elegans is a widely accepted model system for parasitic nematodes, drug screening and developmental studies...

Keywords: Mass spectrometry, nematodes, posttranslational modification, protease

BID, an interaction partner of protein kinase CK2?

Recombinant murine BID protein was used as an in vitro substrate for the CK2 holoenzyme and the catalytic CK2? subunit...

Keywords: Mass spectrometry, phosphorylation, polyamines, protein interaction

PqsA is required for the biosynthesis of 2,4-dihydroxyquinoline (DHQ), a new metabolite produced by Pseudomonas aeruginosa and Burkholderia thailandensis

A new metabolite, 2,4-dihydroxyquinoline (DHQ), was identified in cultures of the bacteria Pseudomonas aeruginosa and Burkholderia thailandensis...

Keywords: 2,4-dihydroxyquinoline, 4-hydroxy-2-alkylquinolines, Mass spectrometry, natural product, Pseudomonas aeruginosa, PqsA

Position of chromatographic techniques in screening for detection of drugs or poisons in clinical and forensic toxicology and/or doping control

This paper reviews chromatographic screening procedures for simultaneous detection of several drug classes relevant to clinical and forensic toxicology or doping control in urine or blood using gas chromatography-mass spectrometry (GC-MS), liquid chromatography coupled with a diode-array detector (LC-DAD) or a mass spectrometer (LC-MS)...

Keywords: chromatography, diode-array detection, drugs, Mass spectrometry, screening

Preanalytical and analytical variation of surface-enhanced laser desorption-ionization time-of-flight mass spectrometry of human serum

Background: Surface-enhanced laser desorption-ionization time-of-flight (SELDI-TOF) mass spectrometry of human serum is a potential diagnostic tool in human diseases...

Keywords: Mass spectrometry, proteomics, surface-enhanced laser desorption-ionization time-of-flight(SELDI-TOF), serum protein peaks

Quantitative determination of erythrocyte folate vitamer distribution by liquid chromatography-tandem mass spectrometry

Background: Given the role of folate in many disorders, intracellular distribution of folate vitamers is of potential clinical importance...

Keywords: erythrocyte, folate, folate vitamer, liquid chromatography, Mass spectrometry

How to comprehensively analyse proteins and how this influences nutritional research

Proteomics, the comprehensive analysis of a protein complement in a cell, tissue or biological fluid at a given time, is a key platform within the “omic” technologies that also encompass genomics (gene analysis), transcriptomics (gene expression analysis) and metabolomics (metabolite profiling)...

Keywords: biomarker, health, Mass spectrometry, nutrition, proteomics

Formic and acetic acids in archaeological wood. A comparison between the Vasa Warship, the Bremen Cog, the Oberländer Boat and the Danish Viking Ships

A method has been developed to analyse and quantify formic and acetic acids in archaeological and fresh wood...

Keywords: Acetic Acid, analysis, archaeological wood, formic acid, SPME, gas chromatography, Mass spectrometry

Tandem-Massenspektrometrie in der therapeutischen Arzneimittelspiegel-Bestimmung: Praxis und Tücken der Anwendung / Tandem mass spectrometry in drug monitoring: experience and pitfalls in application

Durch ihre zunehmende Anwenderfreundlichkeit und hohe Sensitivität sowie Spezifität findet die Flüssigkeitschromatographie-gekoppelte (Tandem-) Massenspektrometrie (LC/MS/MS) immer weitere Verbreitung in der Klinischen Chemie und der therapeutischen Arzneimittelspiegel-Bestimmung...

Keywords: Fehlerquellen, Massenspektrometrie, Matrixeffekt, Routineanalytik, Validation, Mass spectrometry, matrix effect, routine analysis, source of error

Synthesis, thermal, spectroscopic and structural properties of di(aqua)bis(N, N′-dimethylethylenediamine-κ²N, N′)copper(II) acesulfamate

The complex [Cu(C₄H₁₂N₂)₂(H₂O)₂]²⁺ [(C₄H₄NO₄S)^–]₂, which is the first structurally characterized complex with acesulfamate as the counter anion, has been synthesized and characterized by elemental analyses, magnetic and conductivity measurements, UV/vis, FT-IR and mass spectra...

Keywords: Acesulfamate, N,N-dimethylethylenediamine, Mass spectrometry, Thermal analysis, Single crystal structure analysis

Mass spectrometry and theoretical investigation of di-alkylphosphoric acid–lanthanide complexes

In the framework of nuclear waste reprocessing, separation processes of minor actinides from fission products are developed using liquid–liquid extraction...

Keywords: electrospray ionization, Mass spectrometry, Dialkylphosphoric acid, Lanthanide, Quantum chemistry

Quantitative profiling of folate and one-carbon metabolism in large-scale epidemiological studies by mass spectrometry

Background: Derangements of one-carbon metabolism have been related to the development of chronic diseases...

Keywords: choline, folate, Mass spectrometry, one-carbon metabolism, riboflavin, vitamin B6

Use of SELDI-TOF mass spectrometry for identification of new biomarkers: potential and limitations

Surface-enhanced laser desorption time of flight mass spectrometry (SELDI-TOF-MS) is an important proteomic technology that is immediately available for the high throughput analysis of complex protein samples...

Keywords: biomarker identification, Mass spectrometry, protein profiling, proteomics, surface-enhanced laser desorption time of flight mass spectrometry (SELDI-TOF-MS)