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Items for "isothermal titration calorimetry"

Nucleotide binding and filament assembly of recombinant yeast septin complexes

Septins are filament-forming GTPases involved in cytokinesis and cortical organization...

Keywords: bi-cistronic, co-expression, fluorescence titration, GTP, isothermal titration calorimetry, Saccharomyces cerevisiae

07/2005 | Biological Chemistry, Walter de Gruyter
Comparative thermodynamic analysis of cyclic nucleotide binding to protein kinase A

We have investigated the thermodynamic parameters and binding of a regulatory subunit of cAMP-dependent protein kinase (PKA) to its natural low-molecular-weight ligand, cAMP, and analogues thereof...

Keywords: cAMP-dependent protein kinase, isothermal titration calorimetry, protein-ligand interaction, surface plasmon resonance

02/2007 | Biological Chemistry, Walter de Gruyter