Items for "immunoassay"

A sensitive proximity ligation assay for active PSA

Prostate-specific antigen (PSA) is a widely used marker for prostate cancer. The utility of PSA tests is limited by their inability to differentiate prostate cancer from non-malignant conditions such as benign prostatic hyperplasia and prostatitis...

Keywords: immunoassay, peptide, prostate cancer, prostate-specific antigen

Position of immunological techniques in screening in clinical toxicology

There is a continuing increase in the use of immunological techniques in the field of clinical toxicology...

Keywords: clinical toxicology, drug screening, immunoassay, near patient testing

Analytical performance of a new two-step ADVIA Centaur^® estradiol immunoassay during ovarian stimulation

Measurement of estradiol is useful in women undergoing ovarian stimulation for in vitro fertilization and embryo transfer (IVF-ET)...

Keywords: estradiol, immunoassay, ovarian stimulation, sensitivity

Variations in assay protocol for the Dako cystatin C method may change patient results by 50% without changing the results for controls

Background: Cystatin C is increasingly used as a glomerular filtration marker, but so far only a few companies produce most of the cystatin C reagents suited for turbidimetry or nephelometry use in clinical laboratories.

Methods: We studied different protocols for measuring cystatin C on an Architect ci8200 system using cystatin C reagents from Dako (Glostrup, Denmark)...

Keywords: cystatin C, erroneous results, glomerular filtration ratio, immunoassay, Kidney function, recombinant proteins, turbidimetry

Immunochemical quantification of free immunoglobulin light chains from an analytical perspective

Immunoglobulin light chains are components of antibodies, but some exist in a free form in serum and urine as a result of their excess production over heavy chains...

Keywords: antibody, Bence Jones protein, free immunoglobulin light chain, immunoassay, standardization

Association of aminothiols with the clinical outcome in hemodialysis patients: comparison of chromatography and immunoassay for homocysteine determination

Background: Controversial results on hyperhomocysteinemia and cardiovascular risk in hemodialysis (HD) could be due in part to the methodology used for homocysteine (Hcy) determination.

Objective: The aim of this study was to compare the influence of the method used for Hcy determination (chromatography or immunoassay) with regard to the association of Hcy with cardiovascular mortality rate in HD patients in a 3-year prospective study.

Methods: A total of 162 patients undergoing HD were included in a cohort study...

Keywords: aminothiols, cardiovascular, chromatography, Hemodialysis, homocysteine, immunoassay

Drug screening: Actual status, pitfalls and suggestions for improvement/Drogenscreening: Gegenwärtiger Stand, Fehlermöglichkeiten und Verbesserungsvorschläge

Immunoassays for drug screening are often regarded as procedures which are easily performed and interpreted...

Keywords: Bestätigungsanalyse, Empfindlichkeit, Immunoassay, Qualitätssicherung, Spezifizität, Teststreifen, Verfälschungsstoffe, Verlaufskontrolle

Quantification of vascular endothelial growth factor, interleukin-8, and basic fibroblast growth factor in plasma of cancer patients and healthy volunteers – comparison of ELISA and microsphere-based multiplexed immunoassay

Background: Vascular endothelial growth factor (VEGF), interleukin-8 (IL-8) and basic fibroblast growth factor (basic FGF) are angiogenic growth factors which may be useful as biomarkers in drug development, where they could give early information on the antiangiogenic activity of novel anticancer compounds.

Methods: We compared two commercially available assays, enzyme linked immunosorbent assay (ELISA) and a multiplexed bead-based immunoassay (xMAP), for the quantification of these factors in plasma samples from more than 100 cancer patients and healthy individuals.

Results: For VEGF and IL-8, but not for basic FGF, xMAP was more sensitive than the respective ELISA...

Keywords: Angiogenesis, biomarker, Cancer, growth factor, immunoassay

Evaluation of the DCA Vantage analyzer for HbA_1c assay

Background: Measurement of HbA_1c is key in monitoring diabetic patients in both laboratories and clinical units, where HbA_1c results are used as part of patient education.

Methods: We have evaluated the DCA Vantage^®, a new device for immunological assay of HbA_1c...

Keywords: Diabetes mellitus, Evaluation, HbA1c, immunoassay, patient outcome, point-of-care testing