Items for "high-density lipoprotein (HDL)"

Circulating levels of nitrated apolipoprotein A-I are increased in type 2 diabetic patients

Recent work has shown that high-density lipoprotein (HDL) isolated from human atherosclerotic lesions and the blood of patients with established coronary artery disease contains elevated levels of 3-nitrotyrosine and 3-chlorotyrosine...

Keywords: diabetes, high-density lipoprotein (HDL), macroangiopathy, myeloperoxidase, nitrosative stress, peroxynitrite

Use of the TLX ultracentrifuge for the isolation of different density lipoproteins and effects of freeze/thawing of human plasma before ultracentrifugation

Background: Isolation of different density lipoproteins by ultracentrifugation can require lengthy centrifugation times and freeze/thawing of plasma may influence recovery.

Methods: We isolated a range of lipoproteins using a preparative ultracentrifuge and the TLX micro-ultracentrifuge and determined the effect of freeze/thawing of plasma beforehand.

Results: In fresh plasma, there was no significant difference in results for small-dense low-density lipoprotein apolipoprotein B (LDL apoB) (density >1.044 g/mL) or cholesterol at density >1.006 g/mL...

Keywords: high-density lipoprotein (HDL), lipoproteins, non-HDL cholesterol, small-dense low-density lipoprotein, ultracentrifugation, very low-density lipoprotein