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Items for "hemoglobin"

Blood transfusions in athletes. Old dogmas, new tricks

Blood doping consists of any illicit means used to increase and optimize oxygen delivery to the muscles and includes blood transfusions, administration of erythropoiesis-stimulating substances, blood substitutes, natural or artificial altitude facilities, and innovative gene therapies...

Keywords: antidoping testing, blood doping, blood transfusion, hemoglobin, sports

12/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Influence of human haptoglobin polymorphism on oxidative stress induced by free hemoglobin on red blood cells

Background: An in vitro study was conducted to determine whether haptoglobin phenotypes differed in their protective effect against oxidative stress induced by extracellular hemoglobin on red blood cells.

Methods: Conjugated dienes and thiobarbituric acid-reactive substances (TBARS) were determined in human red blood cell membranes in the presence of hemoglobin and various concentrations of each type of purified haptoglobin...

Keywords: haptoglobin phenotypes, hemoglobin, hemolysis, oxidative stress, red blood cell membranes

05/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Role of methionine residues of albumin in T-R conversion of hemoglobin

Background: Hemoglobin (Hb) has peroxidase (POD)-like activity. We found that the addition of albumin to human Hb purified from venous erythrocytes increased its POD-like activity.

Methods: We treated human Hb with a Blue-Toyopearl column of immobilized albumin, compared the treated Hb and native Hb for POD-like activity, the level of Hb-bound 2,3-diphosphoglycerate (2,3-DPG), and the absorption spectrum, and found that treatment with albumin released 2,3-DPG from Hb, resulting in a tense-relaxed (T-R) conversion and increased POD-like activity.

Results: The addition of human, mouse, rat, or bovine albumin to human Hb increased its POD-like activity...

Keywords: Albumin, domain, domain I, hemoglobin, Methionine, POD-like activity

09/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Strong interference of hemoglobin concentration on CSF total protein measurement using the trichloroacetic acid precipitation method

Background: Among other methods, trichloroacetic acid precipitation is used to quantify total protein in cerebrospinal fluid (CSF).

Methods: We analyzed the influence of hemoglobin on total protein concentration assayed by the trichloroacetic acid method and compared the results to the benzethonium chloride method.

Results: Four CSF samples were spiked with different amounts of hemoglobin, leading to overestimation of protein concentration when assayed by the trichloroacetic acid method...

Keywords: cerebrospinal fluid, hemoglobin, interference, protein, trichloroacetic acid

01/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Impact of high maternal hemoglobin at first antenatal visit on pregnancy outcomes: a cohort study

Aim: To determine whether high maternal hemoglobin (Hb) at first antenatal visit is associated with adverse pregnancy outcomes.

Methods: A retrospective cohort study was conducted in 920 singleton pregnancies who started their antenatal booking in the first trimester (?14 weeks' gestation)...

Keywords: Antenatal visit, hemoglobin, pregnancy outcomes

03/2008 | Journal of Perinatal Medicine, Walter de Gruyter