Blood doping consists of any illicit means used to increase and optimize oxygen delivery to the muscles and includes blood transfusions, administration of erythropoiesis-stimulating substances, blood substitutes, natural or artificial altitude facilities, and innovative gene therapies...
Keywords: antidoping testing, blood doping, blood transfusion, hemoglobin, sports
12/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Background: An in vitro study was conducted to determine whether haptoglobin phenotypes differed in their protective effect against oxidative stress induced by extracellular hemoglobin on red blood cells.
Methods: Conjugated dienes and thiobarbituric acid-reactive substances (TBARS) were determined in human red blood cell membranes in the presence of hemoglobin and various concentrations of each type of purified haptoglobin...
Keywords: haptoglobin phenotypes, hemoglobin, hemolysis, oxidative stress, red blood cell membranes
05/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Background: Hemoglobin (Hb) has peroxidase (POD)-like activity. We found that the addition of albumin to human Hb purified from venous erythrocytes increased its POD-like activity.
Methods: We treated human Hb with a Blue-Toyopearl column of immobilized albumin, compared the treated Hb and native Hb for POD-like activity, the level of Hb-bound 2,3-diphosphoglycerate (2,3-DPG), and the absorption spectrum, and found that treatment with albumin released 2,3-DPG from Hb, resulting in a tense-relaxed (T-R) conversion and increased POD-like activity.
Results: The addition of human, mouse, rat, or bovine albumin to human Hb increased its POD-like activity...
Keywords: Albumin, domain, domain I, hemoglobin, Methionine, POD-like activity
09/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Background: Among other methods, trichloroacetic acid precipitation is used to quantify total protein in cerebrospinal fluid (CSF).
Methods: We analyzed the influence of hemoglobin on total protein concentration assayed by the trichloroacetic acid method and compared the results to the benzethonium chloride method.
Results: Four CSF samples were spiked with different amounts of hemoglobin, leading to overestimation of protein concentration when assayed by the trichloroacetic acid method...
Keywords: cerebrospinal fluid, hemoglobin, interference, protein, trichloroacetic acid
01/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Aim: To determine whether high maternal hemoglobin (Hb) at first antenatal visit is associated with adverse pregnancy outcomes.
Methods: A retrospective cohort study was conducted in 920 singleton pregnancies who started their antenatal booking in the first trimester (?14 weeks' gestation)...
Keywords: Antenatal visit, hemoglobin, pregnancy outcomes
03/2008 | Journal of Perinatal Medicine, Walter de Gruyter