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Items for "GTPase-activating protein"

Monitoring the real-time kinetics of the hydrolysis reaction of guanine nucleotide-binding proteins

The conversion of guanosine triphosphate (GTP) to guanosine diphosphate (GDP) and inorganic phosphate (Pi) by guanine nucleotide-binding proteins (GNBPs) is a fundamental enzyme reaction in living cells that acts as an important timer in a variety of biological processes...

Keywords: fluorescence reporter, GTPase, GTPase-activating protein, GTP hydrolysis, guanine nucleotide-binding protein, rhodamine, Spectroscopy

11/2005 | Biological Chemistry, Walter de Gruyter
Fluoride complexes of oncogenic Ras mutants to study the Ras-RasGAP interaction

Down-regulation of Ras signalling is mediated by specific GTPase-activating proteins (GAPs), which stimulate the very slow GTPase reaction of Ras by 105-fold...

Keywords: aluminium fluoride, beryllium fluoride, GTPase, GTPase-activating protein, neurofibromin, oncogene, Ras, transition state

09/2008 | Biological Chemistry, Walter de Gruyter