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Items for "G protein-coupled receptor"

Inhibition of sequestration of human B2 bradykinin receptor by phenylarsine oxide or sucrose allows determination of a receptor affinity shift and ligand dissociation in intact cells

Depending on their interaction with intracellular proteins, G protein-coupled receptors (GPCR) often display different affinities for agonists at 37°C...

Keywords: G protein-coupled receptor, internalization, kinin, NPXXY

09/2004 | Biological Chemistry, Walter de Gruyter
C-Terminal fusion of eGFP to the bradykinin B2 receptor strongly affects down-regulation but not receptor internalization or signaling

A functional comparison was made between the wild-type bradykinin B2 receptor (B2wt) and the chimera B2eGFP (enhanced green-fluorescent protein fused to the C-terminus of B2wt), both stably expressed in HEK 293 cells...

Keywords: bradykinin B2 receptor, endocytosis, G protein-coupled receptor, green fluorescent protein

05/2006 | Biological Chemistry, Walter de Gruyter