Items for "functional analysis of separated alleles in yeast (FASAY)"

Preservation of RNA for functional analysis of separated alleles in yeast: comparison of snap-frozen and RNALater^® solid tissue storage methods

Background: The aim of the present study was to compare RNALater^® with the usual method of liquid nitrogen snap freezing as a surrogate mRNA preservation method for functional analysis of separated alleles in yeast (FASAY).

Methods: A total of 81 patients with transitional cell carcinoma of the bladder underwent fresh tissue biopsies directly transferred into RNALater^® and stored at room temperature or at 4°C for increasing time intervals until RNA processing...

Keywords: functional analysis of separated alleles in yeast (FASAY), RNALater®, RNA preservation, snap-frozen