Items for "frozen whole blood"

Quantification of relative changes in specific mRNAs from frozen whole blood – methodological considerations and clinical implications

Background: Based on quantification of relative changes in lipopolysaccharide (LPS)-regulated mRNA transcripts, the present study aimed to establish a robotic method to isolate RNA from stabilized frozen whole blood suitable for gene expression analysis.

Methods: Whole blood (±LPS) was stored in EasyLyse? solution or PAXgene^® tubes (room temperature and ?70°C) for comparison of storage methods, then subjected to robotic isolation of total RNA...

Keywords: frozen whole blood, Gene expression, globin mRNA, PAXgene®, Quality Control, sample size