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fluorescence titration
Items for "fluorescence titration"
Rational engineering of a fluorescein-binding anticalin for improved ligand affinity
The anticalin FluA is an artificial lipocalin with novelspecificity for the fluorescein group, which was engineered from an insect bilin-binding protein by targeted random mutagenesis and selection...
Keywords: circular dichroism, fluorescence titration, hapten, ligand binding, lipocalin, protein design, Spectroscopy
11/2005 | Biological Chemistry, Walter de GruyterNucleotide binding and filament assembly of recombinant yeast septin complexes
Septins are filament-forming GTPases involved in cytokinesis and cortical organization...
Keywords: bi-cistronic, co-expression, fluorescence titration, GTP, isothermal titration calorimetry, Saccharomyces cerevisiae
07/2005 | Biological Chemistry, Walter de GruyterNef protein of human immunodeficiency virus type 1 binds its own myristoylated N-terminus
HIV-1 Nef is a small protein (approx. 25 kDa) that is posttranslationally modified by myristoylation...
Keywords: fluorescence titration, peptide binding, protein conformation
02/2007 | Biological Chemistry, Walter de Gruyter