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Items for "fluorescence resonance energy transfer"

Latent MMP-9 is bound to TIMP-1 before secretion

Expression patterns of matrix metalloproteinase-9 (MMP-9) and its specific inhibitor, tissue inhibitor of metalloproteinases-1 (TIMP-1), are closely correlated with physiological and pathological processes characterized by the degradation and accumulation of the extracellular matrix (ECM)...

Keywords: confocal laser scanning microscopy, fluorescence resonance energy transfer, Matrix metalloproteinase, tissue inhibitor of metalloproteinases

11/2007 | Biological Chemistry, Walter de Gruyter
Rapid detection of the factor XIII Val34Leu (163 G?T) polymorphism by real-time PCR using fluorescence resonance energy transfer detection and melting curve analysis

The Val34Leu polymorphism in the A subunit of blood coagulation factor XIII (FXIII-A) is located in the activation peptide, just three amino acids upstream of the thrombin cleavage site...

Keywords: factor XIII, fluorescence resonance energy transfer, gene polymorphism, real-time PCR

08/2004 | Clinical Chemical Laboratory Medicine, Walter de Gruyter
Enzyme-free signal amplification of analyte in a single closed tube by fluorescent hybridization chain reaction

Background: Hybridization chain reaction (HCR) is an attractive method to amplify the signal of targeted nucleic acids.

Methods: Fluorescent HCR systems, including basic fluorescent HCR and real-time fluorescent HCR, were developed and evaluated in the present study.

Results: Fluorescent HCR but not basic HCR could be performed by enzyme-free signal amplification in a single closed tube...

Keywords: fluorescence resonance energy transfer, nucleic acid amplification, nucleic acid hybridization

10/2008 | Clinical Chemical Laboratory Medicine, Walter de Gruyter