Items for "flow cytometry"

Potential pitfalls of comparative measurements of reticulocytes with flow cytometry and microscopy in prematures and infants

Reticulocyte counting by flow cytometry (Bayer H*3, ADVIA 120) in blood of prematures, infants and children > 1 year of age was compared with microscopic counting under research conditions (9000 counted red blood cells per slide)...

Keywords: flow cytometry, infants, microscopy, neonates, preterms, reticulocytes

Stepwise strategies in analysing haematuria and leukocyturia in screening

The aim of the present work was to compare in a supposed healthy population of 680 subjects several algorithms for positive selection of urine samples requiring microscopic examination for erythrocytes and leukocytes after screening by automated test-strip measurement and particle counting on a Sysmex UF-50? flow cytometer...

Keywords: economic cost, flow cytometry, microscopy, test strip, strategy, urinalysis

Multiplex polymerase chain reaction on FTA cards vs. flow cytometry for B-lymphocyte clonality

Background: Two-colour flow cytometry was compared with multiplex PCR with capillary electrophoresis for clonality determination in specific categories of B-cell lymphoma...

Keywords: B-cell, comparative study, DNA/analysis/isolation and purification, flow cytometry, lymphoma, polymerase chain reaction

Durchflußzytometrische Typisierung der B-Zell Non-Hodgkin-Lymphome / Flow cytometric typing of B-cell Non Hodgkin lymphoma

Zusammenfassung Die durchflußzytometrische Analyse von B-Zell-Lymphomen ist neben der zytologischen Beurteilung und der Histopathologie in der Diagnostik dieser vielfältigen Erkrankungen eine Routineuntersuchung...

Keywords: chronisch lymphatische Leukämie (CLL), diffus grosszelliges B-Zell-Lymphom (DLCL), Flowzytometrie, follikuläres Lymphom (FL), Haarzell-Leukämie (HCL), immunologische Typisierung, lymphoplasmozytisches Lymphom (LPL), Mantelzell-Lymphom (ML), multiples Myelom (MM), nodales Marginalzonenlymphom (NMZL), Non Hodgkin Lymphome (NHL), Prolymphozytenleukämie (PLL), splenomegales Lymphom mit villösen Lymphozyten (SLVL), chronic lymphocytic leukemia (CLL), diffuse large cell lymphoma (DLCL), flow cytometry, follikular lymphoma (FL), hairy cell leukemia (HCL), mantle cell lymphoma (ML), multiple myeloma (MM), nodale marginal zone B-lymphoma (NMZL), Non Hodgkin lymphoma (NHL), immunological phenotyping, lymphoplasmocytic lymphoma (LPL), prolymphocytic leukemia (PLL), splenic marginal zone lymphoma (SMZL)

Differentiation of anti-platelet antibodies using microspheres and flow cytometry Einsatz von Mikrosphären und Durchflusszytometrie für die Differenzierung von Antithrombozyten-Antikörpern

Background: Allo- and autoantibodies against membrane structures of human platelets cause different types of immune thrombocytopenic disorders and require a sensitive and specific assay...

Keywords: Antithrombozyten-Antikörper, Durchflusszytometrie, monoclonal antibody immobilization of platelet antigens (MAIPA), Mehrfachtestung, Mikrosphären

Natürliche Killerzellen und Natürliche Killer-T-Zellen bei Nierentransplantation / Natural killer cells and natural killer-T cells in patients with renal transplantation

Zusammenfassung Die Nierentransplantation als etabliertes Verfahren zur Behandlung des terminalen Nierenversagens birgt trotz hoher klinischer Standards besonders im postoperativen Bereich das Risiko zahlreicher Komplikationen...

Keywords: Durchflusszytometrie, natürliche Killer-T-Zellen, natürliche Killerzellen, transplantation, flow cytometry, natural killer cells, natural killer T-cells

Determination of CD4 lymphocyte percentages using a new simplified and affordable no lyse flow cytometric protocol: a pilot study / Bestimmung der Lymphozyten-abhängigen CD4-Prozentwerte mit Hilfe eines neuen, vereinfachten und kostengünstigen, durchfluss

Although the pathogenesis of HIV infection is independent of age, specific considerations have to be taken into account for the treatment of infants, children and adolescents...

Keywords: CD4, flow cytometry, HIV, paediatric, Durchflusszytometrie, pädiatrisch

Rapid methods for diagnosis of bloodstream infections

Direct detection technologies for pathogenic microorganisms are emerging to be applied in the diagnosis of serious bloodstream infections and infections at sterile body sites, as well as for quality control measures prior to the release of sterile blood products and to ascertain microbial safety of food...

Keywords: bacteraemia, blood culture, flow cytometry, hybridisation, matrix-assisted laser-desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF), microarray, microbial food safety, nucleic acid-based technology (NAT), nucleic acid sequence based amplification (NASBA), PCR, proteomic profiling, sepsis