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Items for "ELISA"

Relation between ADAMTS13 activity and ADAMTS13 antigen levels in healthy donors and patients with thrombotic microangiopathies (TMA)

We have established a new, enzyme-linked immunosorbent assay (ELISA) for the detection of ADAMTS13 antigen using purified polyclonal rabbit anti-human ADAMTS13 IgG...

Keywords: ADAMTS13, ADAMTS13-antigen, ELISA, TTP, HUS

00/0000 | Thrombosis and Haemostasis, Schattauer

Evaluation of the technical performance of novel holotranscobalamin (holoTC) assays in a multicenter European demonstration project

A commercially available holotranscobalamin (holo-TC) radioimmunoassay (RIA) (Axis-Shield, Dundee, Scotland) was evaluated in four laboratories and compared with a holoTC ELISA run in one laboratory...

Keywords: ELISA, holotranscobalamin (holoTC), radioimmunoassay (RIA), Validation

10/2005 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Buprenorphine detection in biological samples

Buprenorphine is a narcotic analgesic used in the treatment of moderate-to-severe pain...

Keywords: alternative matrices, buprenorphine, ELISA

12/2005 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

High-affinity antibodies in a new immunoassay for plasma tissue factor: reduction in apparent intra-individual variation

Tissue factor, the main initiator of blood coagulation, is shed into plasma by blood cells and endothelium...

Keywords: antibody affinity, ELISA, Thromboplastin, Tissue factor

12/2005 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Autoimmune bullous disorders

Bullous skin diseases represent a group of organ-specific autoimmune disorders characterised by binding of circulating autoantibodies to adhesion molecules of the epidermis and the dermo-epidermal basement membrane zone...

Keywords: adhesion molecules, autoantibodies, basement membrane zone, bullous skin diseases, desmosomes, ELISA, immunofluorescence, sodium chloride-split skin

02/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Detection of antibodies against 60-, 65- and 70-kDa heat shock proteins in paediatric patients with various disorders using Western blotting and ELISA

Background: We examined antibodies against 60-, 65- and 70-kDa heat shock proteins (HSPs) in paediatric healthy individuals, patients with juvenile idiopathic arthritis (JIA) and those undergoing allogeneic stem-cell transplantation for various malignant and non-malignant diseases.

Methods: Western blotting and ELISA were used to examine HSP-directed humoral immune responses.

Results: Using ELISA we detected anti-Hsp60, -Hsp65 and -Hsp70 IgG antibodies in patient sera before, during and after conditioning and at all post-transplant times, as well as in JIA patients and controls...

Keywords: antibody, ELISA, heat shock protein, juvenile idiopathic arthritis, stem cell transplantation, Western blotting

04/2006 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Anti-thyroid-stimulating hormone receptor antibodies determined by second-generation assay

Background: The aim of our study was to determine the frequency of anti-thyroid-stimulating hormone (TSH) receptor antibodies (TRAb) in Tunisian patients with Graves' disease (GD) and to compare the validity of TRAb to that of thyroperoxidase (TPO-Ab) and thyroglobulin antibodies (TG-Ab).

Methods: ELISA was used to determine the frequency of TRAb, TPO-Ab and TG-Ab in sera of 190 patients with GD...

Keywords: ELISA, thyroglobulin antibodies, thyroperoxydase antibodies, TSH receptor antibodies

01/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

HER-2 protein concentrations in breast cancer cells increase before immunohistochemical and fluorescence in situ hybridization analysis turn positive

Background: The level of HER-2/neu in breast cancer cells is normally measured by immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH)...

Keywords: breast cancer, ELISA, ERBB2, fluorescence in situ hybridization, HER-2, immunohistochemistry

02/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Bullöse Autoimmundermatosen Autoimmune bullous disorders

Zusammenfassung

Bullöse Autoimmundermatosen stellen eine Gruppe organspezifischer Autoimmunerkrankungen dar, die pathogenetisch durch die Bindung von zirkulierenden Autoantikörpern an Adhäsionsstrukturen der Epidermis bzw...

Keywords: Adhäsionsstrukturen, Autoantikörper, Basalmembranzone, Bullöse Autoimmundermatosen, Desmosomen, ELISA, Immunfluoreszenz, Kochsalz-Spalthaut, autoantibodies, adhesion molecules, basement membrane zone, bullous skin diseases, desmosomes, immunofluorescence, sodium chloride-split skin

08/2005 | LaboratoriumsMedizin, Walter de Gruyter

Potential mammalian reservoirs in a bubonic plague outbreak focus in Mbulu District, northern Tanzania, in 2007

This study investigated mammalian involvement in an outbreak of bubonic plague in Mbulu District, northern Tanzania, in March 2007...

Keywords: bubonic plague, ELISA, rodents, Tanzania

09/2008 | Mammalia, Walter de Gruyter

Cell lines that express membrane-associated DNA for anti-DNA antibody detection

Background: In this study, we evaluated the analytical reliability and clinical sensitivity of two tests (indirect immunofluorescence and ELISA) for anti-DNA antibodies which use cell membrane DNA (cmDNA) as antigen (Wil2 NS lymphoblastoid B cell line).

Methods: We tested 97 sera of patients with systemic lupus erythematosus (SLE) and 140 control sera from healthy subjects or from patients with other systemic autoimmune diseases or infectious diseases...

Keywords: anti-double-stranded DNA (anti-dsDNA), anti-nucleosome, cell membrane DNA, ELISA, indirect immunofluorescence, systemic lupus erythematosus

04/2008 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

An international comparability study to determine the sources of uncertainty associated with a non-competitive sandwich fluorescent ELISA

Background: Immunoassays allow the specific detection and quantitation of biological molecules in complex samples at physiologically relevant concentrations...

Keywords: ELISA, Fluorescence, interferon, Uncertainty

07/2008 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

Matrix metalloprotease-2 and -9 concentration and activity in serum and culture medium samples: a methodological reappraisal

Background: Matrix metalloproteases (MMPs) play an important role in cardiovascular remodeling by degrading the extracellular matrix...

Keywords: ELISA, enzymatic assay, matrix metalloproteases (MMP), MMP serum levels, restenosis

10/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter

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Items for "ELISA"