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Items for "circular dichroism"

A proteinase inhibitor from Caesalpinia echinata (pau-brasil) seeds for plasma kallikrein, plasmin and factor XIIa

Caesalpinia echinata is a tree belonging to the Leguminosae family...

Keywords: amino acid sequence, Caesalpinia echinata, circular dichroism, kinin release, Kunitz-type inhibitor, serine proteinase inhibitor

11/2004 | Biological Chemistry, Walter de Gruyter
Interaction of thiamin diphosphate with phosphorylated and dephosphorylated mammalian pyruvate dehydrogenase complex

Kinetic and binding studies were carried out on substrate and cofactor interaction with the pyruvate dehydrogenase complex from bovine heart...

Keywords: circular dichroism, fluoropyruvate, phosphorylation, pyruvamide

01/2005 | Biological Chemistry, Walter de Gruyter
Rational engineering of a fluorescein-binding anticalin for improved ligand affinity

The anticalin FluA is an artificial lipocalin with novelspecificity for the fluorescein group, which was engineered from an insect bilin-binding protein by targeted random mutagenesis and selection...

Keywords: circular dichroism, fluorescence titration, hapten, ligand binding, lipocalin, protein design, Spectroscopy

11/2005 | Biological Chemistry, Walter de Gruyter
Lysophosphatidic acid affinity chromatography reveals pyruvate kinase as a specific LPA-binding protein

Lysophosphatidic acid is a pleiotropic lipid signalingmolecule that evokes a broad array of cellular responses including proliferation, tumor cell invasion, neurite retraction, cytoskeletal rearrangements and smooth muscle contraction...

Keywords: circular dichroism, enzymatic activity, gel filtration, isothermal calorimetry titration, Mass spectrometry, phospholipid binding

11/2005 | Biological Chemistry, Walter de Gruyter
The precursor of secreted aspartic proteinase Sapp1p from Candida parapsilosis can be activated both autocatalytically and by a membrane-bound processing proteinase

Opportunistic pathogens of the genus Candida produce secreted aspartic proteinases (Saps) that play an important role in virulence...

Keywords: aspartic proteinase, Candida parapsilosis, circular dichroism, His6-tag, zymogen conversion

08/2005 | Biological Chemistry, Walter de Gruyter
A spectroscopic analysis of the interaction between the human regulatory proteins RACK1 and Ki-1/57

Ki-1/57 is a 57-kDa cytoplasmic and nuclear protein associated with protein kinase activity and is hyper-phosphorylated on Ser/Thr residues upon cellular activation...

Keywords: circular dichroism, emission fluorescence, molecular modeling, protein-protein interaction, regulatory proteins, surface tryptophans

05/2006 | Biological Chemistry, Walter de Gruyter
Conformational studies on Arabidopsis sulfurtransferase AtStr1 with spectroscopic methods

Sulfurtransferases/rhodaneses (Str) are enzymes widely distributed in archaea, prokaryota and eukaryota, and catalyze the transfer of sulfur from a donor molecule to a thiophilic acceptor substrate...

Keywords: Arabidopsis thaliana, circular dichroism, 3-mercaptopyruvate, mutagenesis, thiosulfate, tryptic digestion

01/2007 | Biological Chemistry, Walter de Gruyter
Purification, characterization, and molecular gene cloning of an antifungal protein from Ginkgo biloba seeds

A novel basic protein with antifungal activity was isolated from the seeds of Ginkgo biloba and purified to homogeneity...

Keywords: antifungal protein, circular dichroism, Ginkgo biloba, ginkgo seeds, molecular cloning, protease inhibitor

03/2007 | Biological Chemistry, Walter de Gruyter
Conformational changes in bovine lactoferrin induced by slow or fast temperature increases

Lactoferrin (LF) is an iron-binding protein present in several secreted substances, such as milk, and has broad antimicrobial and physiological properties...

Keywords: circular dichroism, fluorescence spectroscopy, heat-induced denaturation, lactoferrin, pasteurization process

08/2008 | Biological Chemistry, Walter de Gruyter