Items for "array extension"

Single zinc-finger extension: enhancing transcriptional activity and specificity of three-zinc-finger proteins

In order to demonstrate that an existing zinc-finger protein can be simply modified to enhance DNA binding and sequence discrimination in both episomal and chromatin contexts using existing zinc-finger DNA recognition code data, and without recourse to phage display and selection strategies, we have examined the consequences of a single zinc-finger extension to a synthetic three-zinc-finger VP16 fusion protein, on transcriptional activation from model target promoters harbouring the zinc-finger binding sequences...

Keywords: array extension, DNA binding, four zinc-finger protein, transactivation, transcription factor