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Items for "activity-based probe"

Labelling of four distinct trophozoite falcipains of Plasmodium falciparum by a cystatin-derived probe

Trophozoite cysteine protease (TCP) activity, isolated from Plasmodium falciparum soluble 100 000 g extracts, displayed native falcipain-1 kinetic parameters towards peptidyl substrates...

Keywords: activity-based probe, affinity labelling, cathepsin, cystatin, 2-D electrophoresis, malaria

04/2005 | Biological Chemistry, Walter de Gruyter
PREPL: a putative novel oligopeptidase propelled into the limelight

The prolyl endopeptidase-like protein PREPL has recently attracted attention because its gene is located within two contiguous gene-deletion syndromes, the 2p21 deletion syndrome and the hypotonia-cystinuria syndrome...

Keywords: activity-based probe, enzyme, FP-biotin, hypotonia-cystinuria syndrome, 2p21 deletion syndrome

07/2006 | Biological Chemistry, Walter de Gruyter
Single-cell resolution imaging of membrane-anchored hepatitis C virus NS3/4A protease activity

The study of host and viral membrane-associated proteases has been hampered due to a lack of in vivo assays...

Keywords: activity-based probe, cell-based assay, Discosoma red fluorescent protein, intracellular proteolysis, juxtamembrane protease, membrane-bound serine protease, viral protease

08/2006 | Biological Chemistry, Walter de Gruyter