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Items for "2-D electrophoresis"

Labelling of four distinct trophozoite falcipains of Plasmodium falciparum by a cystatin-derived probe

Trophozoite cysteine protease (TCP) activity, isolated from Plasmodium falciparum soluble 100 000 g extracts, displayed native falcipain-1 kinetic parameters towards peptidyl substrates...

Keywords: activity-based probe, affinity labelling, cathepsin, cystatin, 2-D electrophoresis, malaria

04/2005 | Biological Chemistry, Walter de Gruyter
Intact growth factors are conserved in the extracellular matrix of ancient human bone and teeth: a storehouse for the study of human evolution in health and disease

For the first time we have extracted, solubilized and identified growth factors, such as insulin growth factor II (IGF-II), bone morphogenetic protein-2 (BMP-2), and transforming growth factor-? (TGF-?), from archaeological compact human bone and tooth dentin dating from the late pre-ceramic pottery Neolithic (late PPNB) and the early Middle Ages...

Keywords: ancient bone and teeth, bone morphogenetic protein-2 (BMP-2), 2-D electrophoresis, human evolution, insulin growth factor II (IGF-II), transforming growth factor-? (TGF-?)

08/2005 | Biological Chemistry, Walter de Gruyter
Electrophoretic separations of cerebrospinal fluid proteins in clinical investigations

The cerebrospinal fluid (CSF) is a key sample in the research for novel molecular biomarkers of neurodegenerative disorders...

Keywords: cerebrospinal fluid, 2-D electrophoresis, dementia, proteomics, matrix assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS), neurodegeneration

04/2007 | Clinical Chemical Laboratory Medicine, Walter de Gruyter