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Nathalie Bardin, Valrie Moal, Francine Anfosso, Laurent Daniel, Philippe Brunet, Jos Sampol, Franoise Dignat George
Soluble CD146, a novel endothelial marker, is increased in physiopathological settings linked to endothelial junctional alteration
CD146, a novel cell adhesion molecule localized at the endothelial junction, is involved in the control of cell-cell cohesion. It is found as a soluble form in conditioned medium of cultured endothelial cells. We developed an ELISA and report for the first time the presence of a soluble form of CD146 (sCD146) in the plasma of healthy subjects. Mean sCD146 values (260 60 ng/ml) were higher in subjects over 50 years and in men. We therefore investigated sCD146 values in patients with chronic renal failure (CRF), a clinical setting associated with junctional alterations. A significant increase in sCD146 was found in patients with CRF matched with controls (457 181 ng/ml versus 288 82 ng/ml respectively, p<0.0001). This increase was corroborated by increased endothelial expression of CD146 on kidney biopsies from patients with CRF. In contrast, in patients with CRF no modulation was observed for the soluble and cell-associated form of CD31, another junctional molecule. Together these data indicate that sCD146 circulates in the plasma of healthy subjects. Modifications of its basal levels could reflect alterations of junctional functions such as vascular permeability.
Thrombosis and Haemostasis, Schattauer
Print ISSN: 0340-6245
Volume: 90, 11/2003
Pages: 915 - 920