Raute Sunder-Plassmann, Sandra Rieger, Georg Endler, Martin Brunner, Markus Müller, Christine Mannhalter
Simultaneous analysis of
MDR1 C3435T, G2677T/A, and C1236T genotypes by multiplexed mutagenically separated PCR
P-Glycoprotein (PGP) encoded by the multi-drug-resistance 1 (
MDR1) gene is a member of theATP-binding cassette (ABC) transporter family, drug-transporting proteins involved in the bioavailability and pharmacokinetics of various drugs. Several single nucleotide polymorphisms (SNPs) in the
MDR1 gene have been identified so far that may influence PGP expression levels and function. Thus, genotyping for
MDR1 polymorphisms and determining specific haplotypes may become an important tool in predicting individual susceptibility to developing drug resistance. We developed a new multiplexed allele-specific PCR method based on the principle of mutagenically separated PCR (MS-PCR) for rapid and reliable simultaneous genoptyping of the C3435T polymorphism in exon 26 of the
MDR1 gene and two additional SNPs (G2677T/A in exon 21 and C1236T in exon 12), which are in linkage disequilibrium with
MDR1 C3435T. The accuracy and reliability of this method was confirmed by sequencing the respective regions in the
MDR1 gene. This newly developed
MDR1 MS-PCR will facilitate fast, accurate and economic analysis of
MDR1 genotypes and will provide important information in optimizing individual therapeutic approaches.
Clinical Chemical Laboratory Medicine, Walter de Gruyter
Print ISSN: 1434-6621
Volume: 43, 04/2005
Pages: 192 - 194
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