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Jochen Metzger, Manfred Blobner, Peter B. Luppa

Sensitive Chemiluminescence Immunoassay for the Determination of Rat Serum ?1-Acid Glycoprotein

We present the establishment of a sensitive immunoassay for the determination of ?₁-acid glycoprotein (orosomucoid, AGP) in rat serum. The assay is based upon antigen capture by surface-immobilized specific polyclonal rabbit anti-AGP antibodies with biotinylated rat AGP (rAGP) as the tracer, and formatted as competitive enzyme immunoassay. Signaling is performed by streptavidin-conjugated horseradish peroxidase. Enzyme activity is quantified by an enhanced chemiluminometric method, allowing the sensitive detection of rAGP serum levels in small sample volumes.

Clinical Chemical Laboratory Medicine, Walter de Gruyter

Print ISSN: 1434-6621
Volume: 39, 06/2001
Pages: 514 - 518

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