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Frank Musshoff, Heike Junker, Burkhard Madea

Rapid Analysis of Parathion in Biological Samples Using Headspace Solid-Phase Micro-Extraction (HS-SPME) and Gas Chromatography/Mass Spectrometry (GC/MS)

A simple and rapid method for the analysis of parathion in biological samples is presented. The method consists of the extraction of parathion from blood samples by headspace solid-phase micro-extraction (SPME), followed by capillary gas chromatography and mass spectrometry detection. The recoveries in the blood samples after addition of ammonium sulphate and sulphuric acid were between 85 % and 89% compared to samples prepared in water. Linearity was established over a concentration range of 0.1–5 ?g/g blood with acceptable coefficients of correlation and limits of detection reached 0.02–0.05 ?g/g. The time for an analysis is 57 minutes for one sample, including the extraction step. In conclusion, HS-SPME in combination with GC/MS is an effective method for the determination and quantification of parathion-ethyl and parathion-methyl in biological material.

Clinical Chemical Laboratory Medicine, Walter de Gruyter

Print ISSN: 1434-6621
Volume: 37, 06/1999
Pages: 639 - 642

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