You are here: Home :: Area NEM :: Medical science :: Human medicine

Michael Vogeser, Xaver Schiel, Ute Spöhrer

Quantification of voriconazole in plasma by liquid chromatography-tandem mass spectrometry

Keywords: liquid chromatography-tandem mass spectrometry, Solid phase extraction, therapeutic drug monitoring, voriconazole

A convenient liquid chromatography-tandem mass spectrometry method for the quantification of the triazole antifungal agent voriconazole in plasma samples is described. Fenbuconazole is used as an internal standard. After protein precipitation, automated solid-phase extraction is applied. Electrospray ionization in the positive mode is used and the following mass transitions are recorded: voriconazole, 350?127; and fenbuconazol, 337?125. The analytical run time is 4 min. The response was linear from 78 to 5000 ?g/L. The total coefficient of variation (n=16) was 12.6% for a low-concentration pool (143 ?g/L), 4.7% for a medium-concentration pool (419 ?g/L), and 5.0% for a high-concentration pool (4304 ?g/L). The method is proposed for future investigations that should be performed to test the hypothesis that therapeutic drug monitoring of voriconazole is clinically useful.

Clinical Chemical Laboratory Medicine, Walter de Gruyter

Print ISSN: 1434-6621
Volume: 43, 07/2005
Pages: 730 - 734

Show full article (external site)

Show all available items of this journal

 

Area NEM