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Floris M. J. Zuijderhoudt, Johannes S. Kamphuis, Willem E. Kluitenberg, Jenneke Dorresteijn-de Bok

Precision and Accuracy of a HPLC Method for Measurement of Fecal Porphyrin Concentrations

We studied precision and accuracy of a HPLC method for determination of porphyrins in feces. A commercial standard solution appeared to contain less coproporphyrin (15%) than stated by the manufacturer. The between-batch coefficients of variation were often below 15% and were higher than the within-batch coefficients. The precision of porphyrin measurements was not influenced by the type of porphyria. Recoveries of added coproporphyrin and protoporphyrin were 90% and 108%; coefficients of variation were 6% and 19%, respectively.

Clinical Chemical Laboratory Medicine, Walter de Gruyter

Print ISSN: 1434-6621
Volume: 40, 10/2002
Pages: 1036 - 1039

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