Andreas Raabe, Olaf Kopetsch, Ulrich Groß, Michael Zimmermann, Peter Gebhart
Measurements of Serum S-100B Protein: Effects of
Storage Time and Temperature on Pre-Analytical
Stability
Measurement of S-100B protein in serum or cerebrospinal
fluid is increasingly used as a molecular marker
of brain damage. However, there is no information
about the pre-analytical in vitro stability of S-100B. We
have investigated whether storing blood samples at
different time periods and conditions affected the
measured levels of S-100B protein. Blood samples
were taken from 29 neurosurgical patients with normal
serum S-100B values and from seven patients
with increased serum S-100B values. Blood samples
were immediately divided into nine aliquots for
measuring S-100B immediately and after 4, 8, 12, and
24 hours, stored at room temperature or at 4 °C.
Measurement of S-100B was performed using the
LIAISON® assay (Byk-Sangtec Diagnostica, Dietzenbach,
Germany). Moreover, in 10 additional patients
the effect of freezing the serum and thawing the sample
after 24 hours was investigated. There were no differences
between the results of S-100B measurements
after storing the sample at different temperatures and
time periods. There was no trend towards higher or
lower values in all three groups. Therefore, blood samples
may be collected as part of the daily clinical routine
without time constraint and even stored overnight
without affecting S-100B serum levels when
measured with the LIAISON® Sangtec® 100 assay.
Clinical Chemical Laboratory Medicine, Walter de Gruyter
Print ISSN: 1434-6621
Volume: 41, 05/2003
Pages: 700 - 703
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