SearchPublisher and Institutes
You are here: Home :: Area NEM :: Medical science :: Veterinary medicine
Brenda Jo Salinardi, J. K. Roush, T. Schermerhorn, K. E. Mitchell
Matrix metalloproteinase and tissue inhibitor of metalloproteinase in serum and synovial fluid of osteoarthritic dogs
Keywords: Matrix metalloproteinase, synovial fluid, serum
To better understand the mechanisms responsible for the pathological processes of osteoarthritis (OA) and topotentially identify a profile of changes that could bepredictive of early OA, matrix metalloproteinase-1(MMP-1) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) in the synovial fluid and serum ofnormal and osteoarthritic dogs were examined. Theconcentration of MMP-1 in the synovial fluid of osteoarthriticdogs (0.62 0.16), as measured by densitometry,was significantly higher than that found incontrol dogs (0.42 0.19) (P = 0.03). The concentrationof MMP-1 in the serum of osteoarthritic dogs(0.74 0.16) was significantly less than that foundin control dogs (0.87 0.08) (P = 0.05). The concentrationof TIMP-2 in the synovial fluid of osteoarthriticdogs (46.2 21.9 ng/ml) was significantlyless than that of control dogs (122.0 66.5 ng/ml)(P = 0.009). The concentration of TIMP-2 in theserum of osteoarthritic dogs (116.2 43.1 ng/ml)was not significantly different than that of control dogs(95.1 94.4 ng/ml) (P = 0.554). In addition, aphospho-tyrosine immunoprecipitation and mass spectrometrywere used to isolate and identify interferonalphain canine synovial fluid.
Veterinary and Comparative Orthopaedics and Traumatology, Schattauer
Print ISSN: 0932-0814
Volume: 19
Pages: 49 - 55