N. Ochsenbein-Kölble, G. Bilic, H. Hall, R. Huch, R. Zimmermann
Inducing proliferation of human amnion epithelial and mesenchymal cells
for prospective engineering of membrane repair
Objective: To prepare a tissue engineering approach to
fetal membrane repair after premature rupture of the
membranes (PROM) by characterizing the proliferation
potential of human amnion epithelial and mesenchymal
cells from preterm and term placenta in primary culture.
Methods: Amnion epithelial and mesenchymal cells
from 15 preterm (23–36 week) and 27 term placentas
collected at cesarean section were separated enzymatically,
characterized immunohistochemically (anti-cytokeratin
18 and anti-E-cadherin, and anti-vimentin, respectively),
and their ratio determined. Proliferation on
tissue culture polystyrene (TCPS) or collagen in one
medium and on TCPS in four different media after
14 days was measured photometrically and compared in
preterm vs. term placenta. For statistical analysis the
Mann-Whitney test was used.
Results: Preterm and term epithelial : mesenchymal cell
ratios were 4.3:1 and 7.8:1. Term epithelial cells proliferated
similarly on TCPS or collagen. Mesenchymal
cells proliferated only with fetal bovine serum (FBS).
Proliferation of term amnion cells in medium containing
FBS, epithelial growth factor (EGF), insulin, transferrin
and triidothyronine(T3) was significantly increased
(p < 0.001) compared with the other three media,
and percentage proliferation was slightly higher in
preterm cells.
Conclusion: Characterization of human amnion epithelial
and mesenchymal cells identified the most potent
proliferation-inducing medium yet. Studies of the
wound-healing potential of these cells are needed, examining
their behavior and proliferation on fibrin microbeads
and other extracellular matrixes as the next
step towards engineering membrane repair in PROM.
Journal of Perinatal Medicine, Walter de Gruyter
Print ISSN: 1619-3997
Volume: 31, 07/2003
Pages: 287 - 294
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