U. Aufderhaar, W. Holzgreve, E. Danzer, A. Tichelli, C. Troeger, D. V. Surbek
The impact of intrapartum factors on umbilical cord blood stem cell
banking
Aims: Umbilical cord blood can be used as an alternative
source for related and unrelated allogeneic stem cell
transplantation. This study was undertaken to determine
whether intrapartum factors have an influence on the
hematopoietic cell compartment of cord blood.
Methods: Cord blood samples were obtained from
102 normal full-term deliveries for the banking of stem
cells. We analyzed the influence of intrapartum factors
on the count of CD34+ cells, total nucleated cells, colony
forming units and total volume of collection. Fluorescence-activated cell sorting was used to measure
CD34+ cell numbers. Statistical analysis was undertaken
using Pearson correlation test and multiple regression
analysis.
Results: The higher the infants' birthweight the larger
was the volume. A lower arterial umbilical pH and a
larger blood volume resulted in an increased number of
CD34+ cells. A large blood volume, long duration of labor,
lower arterial and venous pH were correlated with
more nucleated cells. A higher birthweight, larger blood
volume and lower arterial pH resulted in an increased
number of colony forming units.
Conclusions: Some intrapartum factors have an impact
on the characteristics of collected cord blood cells. Stress
during delivery may influence the number of hematopoetic
cells, through altered cytokine production. This
knowledge may facilitate the selection of optimal cord
blood samples for unrelated banking and the early discarding
of suboptimal cord blood samples thus resulting
in the saving of costs related to expensive further processing.
Journal of Perinatal Medicine, Walter de Gruyter
Print ISSN: 1619-3997
Volume: 31, 07/2003
Pages: 317 - 322
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